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Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, <t>4′,6-diamidino-2-phenylindol.</t> (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.
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Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, <t>4′,6-diamidino-2-phenylindol.</t> (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.
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Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, <t>4′,6-diamidino-2-phenylindol.</t> (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.
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Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, <t>4′,6-diamidino-2-phenylindol.</t> (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.
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Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, <t>4′,6-diamidino-2-phenylindol.</t> (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.
4 6 Diamidino 2 Phenylindole, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, <t>4′,6-diamidino-2-phenylindol.</t> (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.
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Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, <t>4′,6-diamidino-2-phenylindol.</t> (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.
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Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, 4′,6-diamidino-2-phenylindol. (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.

Journal: Atherosclerosis Plus

Article Title: Heterozygous transferrin receptor 1 deletion reduces atherosclerotic lesion formation in apolipoprotein E-deficient mice

doi: 10.1016/j.athplu.2025.12.003

Figure Lengend Snippet: Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, 4′,6-diamidino-2-phenylindol. (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.

Article Snippet: Nuclei were counterstained with 4’,6-diamidino-2-phenylindol (catalog # H-1800 , Vector Laboratories).

Techniques: Staining, Immunofluorescence, Western Blot